A Secret Weapon For attune flow cytometer
A Secret Weapon For attune flow cytometer
Blog Article
At that time, the early symptoms were being dismissed as debris, even so the retrospective evaluation demonstrated shared qualities Using the problematic cells in lifestyle. Tracing the an infection served the lab build additional laboratory techniques for screening and security of assay-essential mobile traces.
No matter if you’re a novice or an expert, explore our resources to advance your analysis and expertise in flow cytometry.
Mix acoustic concentrating know-how with significant-parameter Investigation for versatile spectral unmixing and standard workflows.
The award-profitable Invitrogen Attune CytPix Flow Cytometer combines acoustic focusing that has a substantial-pace brightfield digital camera for simultaneous higher throughput flow cytometry and high resolution brightfield imaging.
"A picture just isn't rather worthy of 1000 dots, but it definitely helps you to see illustrations or photos. It is great to discover photographs that correlate with staining. For example, AnnexinV-positive cells which have been morphologically diverse than healthful cells, or CD14+ monocytes which have been larger sized and a lot more textured than CD3+/CD4+/CD14- lymphocytes.
In this article, a highly trained person has gated singlets confidently. Just after evaluating the guide singlet gate, the CytPix graphic-derived parameter ParticleCount reveals this gate consists of in excess of 4% aggregates.
Optimizing gating method. Even strong handbook singlet gating is error-vulnerable and stays a subjective decision point in almost all flow cytometry assays. Imaging can be used to substantiate and regulate gates to include only single cells of fascination.
Our scientists will style panels for yourself as many times you'll need—gratis, no product obtain essential.
Early indications of this contamination had originally been dismissed as debris. Backgating to find new populations. Backgating imaged cells over the Attune CytPix Flow Cytometer also means that you can use morphological features to find out attention-grabbing subpopulations that might not be evident from flow cytometry knowledge by yourself.
Buyers can watch celebration photos in serious-time to verify sample excellent, enhance protocols, and history the morphology of every cell population identified for Evaluation. Imaging capture also Gains from acoustic focusing, which helps to position the cells making sure that a pointy, centered picture is attained.
AI-driven automated impression Investigation While using the Attune Cytometric Software translates event attributes into distinctive morphology parameters which might be combined with common fluorescence and scatter parameters to expose very reproducible inhabitants variations and exceptional subsets.
Software Be aware: Automatic image Assessment decreases user-to-user variability in flow cytometry gating methods
Designed-in dimensionality reduction instruments increased resolution into populations. Fluorescently gated populations again-gated onto UMAP plot to substantiate click here envisioned inhabitants resolution in lysed whole human blood. Consumer can see gatherings from each cluster for visual confirmation of mobile phenotypes.
Versatile payment plan from Agilent Fiscal Options permit you to get the critical instrumentation you would like for instant challenge, or to improve your lab.
Gating on these prolonged parameters allows you to promptly and accurately recognize populations of desire and make sure gating system with little or no handbook critique. The Attune software package provides usage of condition-of-the-artwork machine Discovering analysis tools in an expertly built user interface, making it extremely easy to use.
Because the cell line had Earlier been analyzed about the Attune CytPix Flow Cytometer, the scientists went back to the photographs and ended up capable to document the microbial infection no less than 5 days before.
The Attune CytPix Flow Cytometer permits users to visualize samples throughout acquisition, confirm the precision of gating methods, and guidance outcomes with reputable documentation for publication needs.
What seemed to be a homogeneous population is in fact additional varied—an insight that ought to be thought of when interpreting success.